Preparación de abstracts y posters para congresos Alfredo Prieto Martín Profesor Asociado de Inmunología Coordinador del programa de doctorado en Inmunología,

Presentación del tema: "Preparación de abstracts y posters para congresos Alfredo Prieto Martín Profesor Asociado de Inmunología Coordinador del programa de doctorado en Inmunología,"— Transcripción de la presentación:

1 Preparación de abstracts y posters para congresos Alfredo Prieto Martín Profesor Asociado de Inmunología Coordinador del programa de doctorado en Inmunología, Mención de calidad MECD Universidad de Alcalá alfredo.prieto@uah.es http://www.alfredoprieto.tk alfredo.prieto@uah.es Cómo se hace un abstract para un congreso Se escoge un título Se enumera a los autores Introducción Background Objetivo Material y métodos Resultados Conclusiones Si el congreso es nacional es seguro que te lo aceptarán al menos como poster. Uso de PP para hacer un poster a partir de un simple abstract Configurar pagina para poner las dimensiones de ancho y alto ej. 90 x 100 Escoger fondo, poner el zoom en ajustar Cortar y pegar las distintas partes del abstract (Titulo y autores, introducción, materiales y métodos, resultados, y conclusiones) en cuadros de texto. Maquetación de los cuadros de texto se organizan en dos o tres columnas como las de los periodicos. Colorear los cuadros de texto usando fondos degradados en dos colores:Colorear los cuadros de texto usando fondos degradados en dos colores: color de relleno> efectos de relleno >efectos de relleno>dos colores color de relleno> efectos de relleno >efectos de relleno>dos colores Añadir iconografia (tablas y figuras) que ilustren los resultadosAñadir iconografia (tablas y figuras) que ilustren los resultados Llevar el archivo de PP a un servicio de reprografía o ilustración científica que disponga de ploter (30 euros en la UAH)Llevar el archivo de PP a un servicio de reprografía o ilustración científica que disponga de ploter (30 euros en la UAH) A new paradigm explains dual effects of IL-2 on decisions to cell growth or apoptosis of T cells studied by CSFE tracking and cell enumeration * A Prieto, * D Díaz, * H Barcenilla, * G Revilla, * P Prieto, *J Monserrat,* V Sualdea, *,§ M Álvarez Mon-Soto * Department of Medicine, CSIC R&D Associated Unit University of Alcalá, Alcalá de Henares, Madrid, Spain. § Immune System Diseases and Oncology Service, University Hospital “Príncipe de Asturias”, Alcalá de Henares, Madrid, Spain. Background: The responses of lymphocytes to mitogens are heterogeneous, they can remain either in resting state or blastify and in each of these states can also initiate apoptosis. Material and methods: We enumerated CSFE labeled T cells from phenotypically defined subsets, monitorized the process of cell decision between growth and apoptosis and studied how exogenous IL- 2 (eIL-2) affects such cell decisions subsequent to mitogen stimulation. The viable and apoptotic cells within resting and blastic cell populations were enumerated 3 days after the policlonal stimulation. For the analysis the undivided cells were classified into 4 categories: viable resting, apoptotic resting, viable blasts and apoptotic blasts. The cells which have suffered cell divisions were classified into viable and apoptotic blasts. Results. At 3 days of culture a high proportion of resting T cells have suffered apoptosis. Some of the blasts suffered apoptosis before their first division. The absolute cell numbers of all subtypes of viable T cells (resting cells, undivided blasts and divided blasts) at 3 days were significantly lower in cultures with eIL-2. At 5 and 7 days of culture eIL-2 increased the number and the viability of the progeny of blasts decreasing their proportion of apoptotic cells within each division. The CSFE tracking demonstrates that eIL-2 allows T cells exceed their third division and continue dividing until five or more divisions. Conclusions: eIL-2 has opposed effects on mature T lymphocytes activated by mitogens before their first division and after it. Firstly it induces apoptosis on them and inhibit their transformation in blasts while later in the blasts inhibits their apoptosis decreasing the proportion of apoptotic cells and induces their growth increasing the number of divisions of the proliferating T cells. So IL-2 is a potent proapoptotic factor for mitogen stimulated mature T lymphocytes and a vigorous growth factor for surviving proliferating blasts.

2 Cómo se hace un abstract para un congreso Se escoge un título Se enumera a los autores Introducción Background Objetivo Material y métodos Resultados Conclusiones Si el congreso es nacional es seguro que te lo aceptarán al menos como poster.

3 Uso de PP para hacer un poster a partir de un simple abstract Configurar pagina para poner las dimensiones de ancho y alto ej. 90 x 100Configurar pagina para poner las dimensiones de ancho y alto ej. 90 x 100 Escoger fondo, poner el zoom en ajustarEscoger fondo, poner el zoom en ajustar Cortar y pegar las distintas partes del abstract (Titulo y autores, introducción, materiales y métodos, resultados, y conclusiones) en cuadros de texto.Cortar y pegar las distintas partes del abstract (Titulo y autores, introducción, materiales y métodos, resultados, y conclusiones) en cuadros de texto. Maquetación de los cuadros de texto se organizan en dos o tres columnas como las de los periodicos.Maquetación de los cuadros de texto se organizan en dos o tres columnas como las de los periodicos.

4 Uso de PP para hacer un poster a partir de un simple abstract Colorear los cuadros de texto usando fondos degradados en dos colores:Colorear los cuadros de texto usando fondos degradados en dos colores: color de relleno> efectos de relleno >efectos de relleno>dos colores color de relleno> efectos de relleno >efectos de relleno>dos colores Añadir iconografia (tablas y figuras) que ilustren los resultadosAñadir iconografia (tablas y figuras) que ilustren los resultados Llevar el archivo de PP a un servicio de reprografía o ilustración científica que disponga de ploter (30 euros en la UAH)Llevar el archivo de PP a un servicio de reprografía o ilustración científica que disponga de ploter (30 euros en la UAH)

5 A new paradigm explains dual effects of IL-2 on decisions to cell growth or apoptosis of T cells studied by CSFE tracking and cell enumeration * A Prieto, * D Díaz, * H Barcenilla, * G Revilla, * P Prieto, *J Monserrat,* V Sualdea, *,§ M Álvarez Mon-Soto * Department of Medicine, CSIC R&D Associated Unit University of Alcalá, Alcalá de Henares, Madrid, Spain. § Immune System Diseases and Oncology Service, University Hospital “Príncipe de Asturias”, Alcalá de Henares, Madrid, Spain. Background: The responses of lymphocytes to mitogens are heterogeneous, they can remain either in resting state or blastify and in each of these states can also initiate apoptosis. Material and methods: We enumerated CSFE labeled T cells from phenotypically defined subsets, monitorized the process of cell decision between growth and apoptosis and studied how exogenous IL-2 (eIL-2) affects such cell decisions subsequent to mitogen stimulation. The viable and apoptotic cells within resting and blastic cell populations were enumerated 3 days after the policlonal stimulation. For the analysis the undivided cells were classified into 4 categories: viable resting, apoptotic resting, viable blasts and apoptotic blasts. The cells which have suffered cell divisions were classified into viable and apoptotic blasts. Results. At 3 days of culture a high proportion of resting T cells have suffered apoptosis. Some of the blasts suffered apoptosis before their first division. The absolute cell numbers of all subtypes of viable T cells (resting cells, undivided blasts and divided blasts) at 3 days were significantly lower in cultures with eIL-2. At 5 and 7 days of culture eIL-2 increased the number and the viability of the progeny of blasts decreasing their proportion of apoptotic cells within each division. The CSFE tracking demonstrates that eIL-2 allows T cells exceed their third division and continue dividing until five or more divisions. Conclusions: eIL-2 has opposed effects on mature T lymphocytes activated by mitogens before their first division and after it. Firstly it induces apoptosis on them and inhibit their transformation in blasts while later in the blasts inhibits their apoptosis decreasing the proportion of apoptotic cells and induces their growth increasing the number of divisions of the proliferating T cells. So IL-2 is a potent proapoptotic factor for mitogen stimulated mature T lymphocytes and a vigorous growth factor for surviving proliferating blasts.

6 A NEW PARADIGM EXPLAINS DUAL EFFECTS OF IL-2 ON DECISIONS TO CELL GROWTH OR APOPTOSIS OF T CELLS STUDIED BY CFSE TRACKING AND CELL ENUMERATION * A Prieto, * D Díaz, * H Barcenilla, * G Revilla, * P Prieto, *J Monserrat,* V Sualdea, *,§ M Álvarez Mon-Soto * A Prieto, * D Díaz, * H Barcenilla, * G Revilla, * P Prieto, *J Monserrat,* V Sualdea, *,§ M Álvarez Mon-Soto * Department of Medicine, CSIC R&D Associated Unit University of Alcalá, Alcalá de Henares, Madrid, Spain. § Immune System Diseases and Oncology Service, University Hospital “Príncipe de Asturias”, Alcalá de Henares, Madrid, Spain. BACKGROUND The responses of lymphocytes to mitogens are heterogeneous, they can remain either in resting state or blastify and in each of these states can also initiate apoptosis. MATERIAL AND METHODS We enumerated CFSE labeled T cells from phenotypically defined subsets, monitorized the process of cell decision between growth and apoptosis and studied how exogenous IL-2 (eIL-2) affects such cell decisions subsequent to mitogen stimulation. The viable and apoptotic cells within resting and blastic cell populations were enumerated 3 days after the policlonal stimulation (Figure 1). At 5 and 7 days of culture eIL-2 increased the percentage of viability of the progeny of blasts decreasing their proportion of apoptotic cells within each division. The CFSE tracking demonstrates that eIL-2 allows T cells exceed their third division and continue dividing until five or more divisions. CONCLUSIONS eIL-2 has opposed effects on mature T lymphocytes activated by mitogens before their first division and after it. Firstly it induces apoptosis on them and inhibit their transformation in blasts while later in the blasts inhibits their apoptosis decreasing the proportion of apoptotic cells and induces their growth increasing the number of cell divisions. eIL-2 has opposed effects on mature T lymphocytes activated by mitogens before their first division and after it. Firstly it induces apoptosis on them and inhibit their transformation in blasts while later in the blasts inhibits their apoptosis decreasing the proportion of apoptotic cells and induces their growth increasing the number of cell divisions. IL-2 is a potent proapoptotic factor for mitogen stimulated mature T lymphocytes and a vigorous growth factor for surviving proliferating blasts. IL-2 is a potent proapoptotic factor for mitogen stimulated mature T lymphocytes and a vigorous growth factor for surviving proliferating blasts. Figure 4. Effect of IL-2 on proliferación/apoptosis decision in T cell progeny We represent the absolute number of viable (  ) and apoptotic cells (  ) of polyclonally estimulated T cells along serial cell divisions measured by their CFSE labelling.. Figure 2. Absolute number of surviving cells after three days of culture with PHA in abcense (  ) or presence (  ) of eIL-2. Cell Stage RESTING T CELLS UNDIVIDED BLASTS DIVIDED BLAST Viable Cells / Well 0 2000 4000 6000 8000 10000 Number of cell divisions 1234 5 or more Apoptotic blasts Viable blasts -40000 0 40000 80000 120000 160000 200000 RESULTS At 3 days of culture, the absolute cell numbers of all subtypes of viable T cells (resting cells, undivided blasts and divided blasts) were significantly lower in cultures with eIL-2 (Fig. 2). Figure 1. Study of viability in resting T cells and T blasts in each cell division For the analysis the undivided cells were classified into 4 categories: viable resting, apoptotic resting, viable blasts and apoptotic blasts. The cells which have suffered cell divisions were classified into viable and apoptotic blasts Figure 3. Effect of IL-2 on proliferación/apoptosis decision in T cell progeny We represent the percentages of viable (  ) and apoptotic cells (  ) of polyclonally estimulated T cells along serial cell divisions measured by their CFSE labelling.. At 5 and 7 days of culture eIL-2 increased the number and the viability of the progeny of blasts decreasing their proportion of apoptotic cells within each division. The CFSE tracking demonstrates that eIL-2 allows T cells exceed their third division and continue dividing until five or more divisions.