Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

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Transcripción de la presentación:

Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients Juan Carlos Ramírez, Carolina Inés Cura, Otacilio da Cruz Moreira, Eliane Lages-Silva, Natalia Juiz, Elsa Velázquez, Juan David Ramírez, Anahí Alberti, Paula Pavia, María Delmans Flores-Chávez, Arturo Muñoz-Calderón, Deyanira Pérez-Morales, José Santalla, Paulo Marcos da Matta Guedes, Julie Peneau, Paula Marcet, Carlos Padilla, David Cruz-Robles, Edward Valencia, Gladys Elena Crisante, Gonzalo Greif, Inés Zulantay, Jaime Alfredo Costales, Miriam Alvarez-Martínez, Norma Edith Martínez, Rodrigo Villarroel, Sandro Villarroel, Zunilda Sánchez, Margarita Bisio, Rudy Parrado, Lúcia Maria da Cunha Galvão, Antonia Cláudia Jácome da Câmara, Bertha Espinoza, Belkisyole Alarcón de Noya, Concepción Puerta, Adelina Riarte, Patricio Diosque, Sergio Sosa-Estani, Felipe Guhl, Isabela Ribeiro, Christine Aznar, Constança Britto, Zaida Estela Yadón, Alejandro G. Schijman The Journal of Molecular Diagnostics Volume 17, Issue 5, Pages 605-615 (September 2015) DOI: 10.1016/j.jmoldx.2015.04.010 Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Comparative analysis of parasitic loads obtained by SatDNA (open circles) and kDNA (open triangles) qPCR assays for quantifiable samples from CD patients. Distribution of parasitic loads on the basis of the CD phase (A), the clinical status (B), and T. cruzi DTUs (C) of chronic CD patients. ∗∗P < 0.01. CD, Chagas disease; DTU, discrete typing unit; kDNA, kinetoplastid DNA; par. eq./10 mL: parasite equivalents in 10 mL of blood; qPCR, quantitative real-time PCR; SatDNA, Satellite DNA. The Journal of Molecular Diagnostics 2015 17, 605-615DOI: (10.1016/j.jmoldx.2015.04.010) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Bland-Altman bias (difference) plot analysis as a measure of the degree of agreement between the quantifiable results obtained by SatDNA and kDNA qPCR assays for samples from Chagas disease patients. kDNA, kinetoplastid DNA; par. eq./10 mL, parasite equivalents in 10 mL of blood; qPCR, quantitative real-time PCR; SatDNA, Satellite DNA. The Journal of Molecular Diagnostics 2015 17, 605-615DOI: (10.1016/j.jmoldx.2015.04.010) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions