Accesos directos del teclado Fig. 1 A B C Em Comandos de Archivo Ctrl + O Abrir... Ctrl + Alt + Shift + G Ver historial de revisión Ctrl + S Descargar Comandos de edición Ctrl + Alt + M Comentario Ver comandos Ctrl + Shift + F Controles compactos Aplicaciones Ctrl + / Accesos directos del teclado Accesos directos del teclado Ver todos los accesos directos en el Centro de ayuda | Cerrar pHi FAU [Ca2+]i
Fig. 2 A B Zn2+ 5 mV 20 s 0.05 (0.9 pM) 0.1 (1.9 pM) -5 0.1 (1.9 pM) -5 Δ Hyperpolarization (mV) -10 -15 0.5 (9.3 pM) -20 1.0 (19 pM) 0.1 1.0 10 100 Total [Zn2+] (µM) 10 (190 pM) 50 (1.0 nM) 100 (2.1 nM) 300 (9.6 nM) 5 mV 20 s
Normalized hyperpolarization Fig. 3 A B 10 µM Zn2+ 1 0.8 20 s 0.1 F/F0 Control 50KSW 0.6 Normalized hyperpolarization (F/F0) 0.4 * 0.2 50KSW 0ClSW
Normalized Hyperpolarization Fig. 4 A B C Zn2+ Zn2+ Zn2+ Control TEA Control Tolbu Control Glib 10 mV 20 s 20 s 10 mV 20 s 10 mV D E Zn2+ * 2.0 * 1.5 * Normalized Hyperpolarization 1.0 * Control ChTx IbTx 20 s 0.1 F/F0 0.5 0.0 TEA Tolbu Glib IbTx ChTx
Fig. 5 A B * * * 8 cGMP 7 3 Normalized values 2 1 Control speract Control speract KCl + speract Zn2+ KCl + Zn2+ B cAMP 4 * * Normalized values 3 2 1 Control speract KCl + speract Zn2+ KCl + Zn2+
Fig. 6 A B C * * Zn2+ Speract 20 █ Zn2+ █ Speract Control Control 15 Δ Hyperpolarizarion (mV) * 10 5 DTT DTT Control 5 mM DTT 5 mM βMercapto βMercapto Tomada de Figures 190713 Esme βMercapto 10 mV 4 mV 20 s 20 s
Fig. 7 A B E F C D G Speract 5 F/F0 2 s Zn2+ 2 F/F0 2 s 219 350 2 s F Zn2+ C D 2 F/F0 2 s Head Flagellum Zn2+ 205 395 G 0.5 F/F0 2 s 50KSW
Fig.8 A B C pHi [Ca2+]i pHi [Ca2+]i 1.0 0.8 Δ Normalized 0.6 0.4 0.2 Zn2+ Zn2+ █ Control █ 50KSW 0.1 F/F0 0.2 UpH 20 s 20 s C 1.0 0.8 Δ Normalized 0.6 0.4 0.2 0.0 pHi [Ca2+]i
Fig. S1 15 * * 10 Normalized [cAMP] * * 5 1 2.5 5 10 [Zn2+] (µM)
Fig. S2 * 0 Ca2+ Control Δ Hyperpolarization (mV) █ 10 µM Zn2+ █ 100 nM speract 20 15 10 5
Fig. S3 A B AR (%) [Zn2+] (µM) 1.0 0.8 0.6 Normalized AR * 0.4 * * * * FSP Zn2+ 0.8 0.6 Normalized AR * 0.4 * * * * 0.2 * * 0.0 Ni2+ Nife 50KSW 0CaSW NA